Bartolomeo Bacilieri studied Catholic theology at the Episcopal Seminary in Verona and then his doctorate at the Almo Collegio Capranica in Rome as a doctor of theology. After his ordination, which he received in Rome, he was a lecturer at the first Seminar in Verona and was also from 1868 to 1888 the rain. In addition, he was from 1878 until his appointment as bishop canon at the cathedral in Verona.
On June 1, 1888, he was named Pope Leo XIII. Titular Bishop of Nisa in Lycia and the Coadjutor Bishop in the Diocese of Verona. He received his episcopal consecration Mariano Rampolla del Tindaro Cardinal Secretary of State on 10 June of the same year in the chapel of the Collegio Capranica in Rome; Were co-consecrators Alessandro Sanminiatelli Zabarella and Vincenzo Vannutelli. On March 12, 1900, he followed the late Luigi di Canossa according to the Episcopal chair in Verona.
Pope Leo XIII. took him on 15 April 1901 as cardinal priest with the titular church of San Bartolomeo all'Isola to the College of Cardinals. Bacilieri participated in the conclave of 1903 in part, by Pope Pius X chose, as the conclave of 1914, Pope Benedict XV. chosen and the conclave of 1922 that Pope Pius XI. raised to the See of Peter.