Gel electrophoresis#Agarose

The agarose gel electrophoresis is a biochemical and molecular biological method in which nucleic acid strands (RNA or DNA) are separated by gel electrophoresis according to their size and their size and mass by comparison with DNA strands to determine a known size.


Long threads of Agarosepolymeren be solved by briefly boiling in electrophoresis, such as Tris - acetate EDTA buffer ( TAE buffer ), in Tris - boric acid -EDTA buffer ( TBE buffer ) or lithium borate buffer. By cooling a gel of agarose, whose molecules are cross-linked by hydrogen bonds form. The higher the concentration of agarose is, the smaller the pores, which are in the gel.

The samples are mixed prior to electrophoresis sample buffer. The gel acts like a sieve for molecules. Upon application of an electric field causes an ion current of the electrolytes used, attract the negatively charged nucleic acid molecules in the direction of the positive pole through the Gelmaschen, with the smaller molecules are less restrained, therefore move faster through the gel, causing a separation of the strands is made possible by size.

Molar Mass

The number of bases or base pairs is proportional to the chain length and approximately proportional to the respective molecular weight. By comparison with the length of a DNA ladder of DNA or RNA can be determined.