Sperm-mediated gene transfer

Sperm -mediated gene transfer (English sperm -mediated gene transfer, SMGT ) is a method of gene therapy which utilizes the sperm as a vector to produce a transgenic organism. The sperm- mediated gene transfer combines the artificial fertilization by intracytoplasmic sperm injection ( ICSI) with DNA-loaded sperm.

Principle

Sperm can bind in the head region and record ( in subacrosomalen area and in the equatorial segment) DNA. Sperm- mediated gene transfer for the seeds first liquid is removed, to remove the inhibitory factor IF-1. An additional transfection efficiency of recording can be enhanced. Restriction enzymes can increase the integration of transgenic DNA after fertilization of an egg. Loaded with sperm DNA can be introduced by intracytoplasmic sperm injection in oocytes then under artificial insemination. Since there is no insertion of the DNA into the DNA of the sperm, are not necessarily transgenic all daughter cells but is either transgenic completely, transgenic mosaic or not transgenic for a premature degradation of the DNA.