5-Bromo-4-chloro-3-indolyl phosphate

  • (5 -bromo -4-chloro -1H- indol-3 -yl)- dihydrogenphosphate
  • BCIP
  • X - phosphate
  • X -Phos

Colorless solid (disodium salt )

Soluble in water ( disodium salt)

Template: Infobox chemical / molecular formula search available

5-bromo -4-chloro -3- indolyl phosphate ( BCIP ) is an artificial, a chromogenic substrate for the enzyme alkaline phosphatase (AP).

Properties

The enzyme alkaline phosphatase to hydrolyzed indolyl phosphate, 5-bromo -4- chlorindolyl and phosphate. The 5- bromo-4- chlorindolyl is, 4'- dichloroindigo oxidized by oxygen in the air to deep blue dye 5,5 '- dibromo -4.

As BCIP is sparingly soluble in many solvents, either the di-sodium salt (water soluble) or, more commonly, the p -toluidine salt ( soluble in DMF ) are used.

Use

In biochemistry BCIP is used in combination with NBT for sensitive detection of the enzyme alkaline phosphatase (AP ).

CAS registry number PubChem Enzyme substrate (biology) Hydrolysis Nitro blue tetrazolium chloride Digital Object Identifier Analytical Biochemistry X-Gluc
14479
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