Since 1991, Charlotte Becker is active in cycling. In both 2000 as 2001, she was third in the individual pursuit at Junior Track World Championships. In 2004, she won the title of European Champion ( junior ) in the points race; they could repeat this success in 2005. In 2008 she was German champion in the individual pursuit (Elite ) and second in the points race. In 2008 she took on the Track World Championships in Manchester in third place in the team pursuit, together with Verena Jooss and Alexandra Sontheimer.
2006 Charlotte Becker was German champion in the time trial on the road. Inter alia could 2008 and 2010, the road race Cologne Classic (2010 before her sister Christina ) to decide for themselves as well as the overall standings Holland Ladies Tour (2008). In 2009, she finished third in the Giro della Toscana. In 2010 she won her first World Cup victory at the GP Ciudad Valladolid since before Judith Arndt. In addition, it was German Champion 2010 in the road race and in the individual pursuit at the Deutsche Bahn Cup in Cottbus; in the points race, she was vice - champion. In August 2011, she won second place in the overall ranking of the Trophée d'Or Feminin.
By 2009 Charlotte Becker drove for the team in Nuremberg, since 2010 she was a member of the Cervelo Test Team. After the withdrawal of the Cervelo Test Team from cycling, she moved to the 2011 season for Team HTC - High Road and in 2013 the team Argos - Shimano. On May 28, 2013 were mitgteteilt that the contract has been terminated by both sides. For the second half of the 2013 season they went for the British team Wiggle Honda.