Sartori won at the Junior World Championships in 1993 and 1994 respectively the title in one. In his second junior title he won for the first time in adult class when he won the title with the quadruple sculls. After 1995, he won his second world title, followed in 1996 by a weaker year of the double quartet: the crew rowed in the Olympics only to fourth place. 1998 Sartori won his third world title in 2000 then followed the Olympic victory with the quadruple sculls. After the Olympic victory Sartori rowed mostly with Rossano Galtarossa in the double sculls, 2003 they won the silver at the World Championships behind the Frenchman Sébastien Vieilledent and Adrien Hardy. At the Olympic Games 2004, the two French won before the Slovenian title defenders Luka Spik and Iztok Čop, behind Galtarossa and Sartori received the bronze medal.
2006 increased by Sartori Skull to the belt rowing. With the Italian eight, he won silver in 2006 as well as a year later with the coxless four. At the Olympic Games in 2008 Sartori labeled with the quad without but only ranked eleventh. From 2009 Sartori returned to the sculling boats and rowed mostly in Italian sculls. Together with Romano Battisti he qualified in 2012 in Lucerne for the Olympic Games in London, where the two silver in the double sculls won.
2000, after his Olympic victory Alessio Sartori was awarded the title of Commander of the Order of Merit of the Italian Republic.